Talks and Posters Presented by National Biomedical EPR Center Faculty & Staff

 2014

Talks
 

First Adriatic Symposium on Biophysical Approached in Biomedical Studies.  Split, Coatia, August 24-29, 2014
 

James Hyde. New non-adiabatic rapid sweep (NARS) spectroscopic EPR methods.

Jeannette Vasquez Vivar. Tetrahydro-biopterin inhibits superoxide release from glutathionylated-eNOS.

W. Karol Subczynski. High membrane cholesterol content and oxidative stress in health and disease.

Jacek Zielonka. Free radical metabolism and bio-energetic changes in cardiovascular diseases and cancer.

Balaraman Kalyanaraman. Profiling and targeting of cellular bioenergetics. Inhibition of pancreatic cancer cell proliferation.

Jimmy Feix. Membrane structure and dynamics and the development of new chemotherapeutic agent.

Jimmy Feix. Selectivity and membrane interaction of D-amino acid substituted antimicrobial peptides.

 2013

Talks
 

IX International Workshop on EPR in Biology and Medicine.  Krakow, Poland, October 7-10, 2013
 

James Hyde. Segmental acquisition of pure absorption and pure dispersion EPR spectra using time averaging.

Balaraman Kalyanaraman. Targeting bioenergetics, metabolism, and redox mechanism to selectively inhibit pancreatic cancer cell proliferation.

Aaron Kittell. Resolution enhancement by differential power saturation using non-adiabatic rapic sweep (NARS) Electron paramagnetic resonance (EPR) spectroscopy.

Laxman Mainali. W-band EPR: Application to high cholesterol containg membranes.

Jason Sidabras.  Novel resonator design at W-band (94 GHz) for electron paramagnetic resonance spectroscopy.

W. Karol Subczynski.  High cholesterol in model and biological membranes:  Effects on health and disease.

Jeannette Vasquez Vivar.  Mitochondrial targeted nitrones:  Use and limitations in the study of mitochondrial derived free radicals.

Jacek Zielonka.  High throughput screening of inhibitors of NADPH oxidases (Nox) -- a combination of fluorescence, HPLC and EPR assays for reliable identification of novel Nox inhibitors.

 2012

Talks
 

Brian Bennett. Biological EPR: Old Tricks and New Horizons. Montana State University, Bozeman, MT | March 6, 2012.

Brian Bennett. Biological EPR: Old Tricks and New Horizons. University of Montana, Missoula, MT | March 8, 2012.

William E. Antholine. How EPR, electron paramagnetic resonance, contributed to the study of anti-tumor agents such as gallium, bleomycin, triapine. Medical College of Wisconsin Cancer Cell Biology Research Forum | March 22, 2012.

► 4th International Trends in Enzymology Conference: Going Beyond Frontiers, Göttingen, Germany | June 4, 2012
 

Brian Bennett. Using EPR to Probe Zinc Enzymes: Old Tricks and New Horizons.

► 54th Rocky Mountain Conference on Analytical Chemistry, Copper Mountain, CO | July 15-19, 2012
 

Laxman Mainali, J.S. Hyde, and W. K. Subczynski. “Spin-label W-band EPR as a Powerful Tool for Studying Membrane Fluidity Profiles in Samples of Small Volume.” (Invited Award Talk)

► Leading the Way: A Joint Conference of the Advancing a Healthier Wisconsin and the Wisconsin Partnership Programs, Milwaukee, WI | September 27, 2012
 

Brian Bennett. Developing Electron Paramagnetic Resonance Tools for Biomarkers of Neurodegeneration.


Posters
 

► Annual Meeting of the Biophysical Society, San Diego, CA | February 25-29, 2012
 

Laxman Mainali, M. Raguz, and W. K. Subczynski,“Phase boundaries in phosphatidylcholine membranes saturated and oversaturated with Cholesterol.”

Laxman Mainali, J. S. Hyde, and W. K. Subczynski “Membrane fluidity profiles as deduced by saturation-recovery EPR measurements of spin-lattice relaxation times of spin labels: multifrequency approach.”

Dellisanti, C.D., Raspanti, J.M., Grant, V.A., Hanson, S.M., Ghosh, B., Satyshur, K.E., Klug, C.S., Czajkowski, C. Site-directed spin labeling reveals pentameric ligand-gated ion channel gating motions.

► 54th Rocky Mountain Conference on Analytical Chemistry, Copper Mountain, CO | July 15-19, 2012
 

Laxman Mainali, M. Raguz, and W. K. Subczynski. “Application of EPR Spin-Labeling Methods to Intact Lens Membranes

R. R. Mett, J. R. Anderson, J. W. Sidabras, and J. S. Hyde. “Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band: Preliminary Results” (PPTX)

► Annual P41 Directors Meeting, Bethesda, MD | 2012
 

Hyde JS. Non-adiabatic rapid scanning EPR.

 2011

Talks
 

Brian Bennett. Electron Paramagnetic Resonance of Metal Ions: Towards in Vivo Structural Biology. Department of Chemistry, Marquette University, Milwaukee, WI | April 8, 2011.

Brian Bennett. Electron Paramagnetic (Spin) Resonance in Biological Environments. Department of Biological Sciences, University of Wisconsin-Parkside, Kenosha, WI | April 22, 2011.

► Association for Research in Vision and Ophthalmology Annual Meeting 2011, Fort Lauderdale, FL | May 1-5, 2011
 

M. Raguz, I. Krpan, W.J. O’Brien, L. Mainali, W.K. Subczynski.  “Physical Properties of the Cortical and Nuclear Fiber-Cell Plasma Membranes from the Human Eye Lens

► 40th Southeastern Magnetic Resonance Conference, Atlanta, GA | November 4, 2011
 

Brian Bennett, EPR Approaches for Biomedicine at the National Biomedical EPR Center.


Posters
 

► Annual Meeting of the Biophysical Society, Baltimore, MD | March 5-9, 2011
 

Alvarez, F.J., Orelle, C., Klug, C.S., Davidson, A.L. Both lobes of maltose binding protein are engaged in stabilization of the semi-open state of the MalK dimer in the maltose ABC transporter.

Mainali, L., Raguz, M., Subczynski, W. K. Organization and Dynamics of Cholesterol Crystalline Domains using EPR Spin-Labeling.

Mainali, L., Raguz, M., Subczynski, W. K. Discrimination and Characterization of Cholesterol Crystalline Domains using EPR Spin-Labeling: Application to Lens Lipid Membranes.

► Experimental Biology, Washington, DC | April 9-11, 2011
 

Zhuo, Y., Zhan, X., Hanson, S.M., Warthaka, M., Dalby, K.N., Gurevich, V.V., KLUG, C.S. (2011) Identification of ERK2-arrestin-3 interaction sites.

► Nicotinic Acetylcholine Receptors 2011, Cambridge, UK | May 18-21, 2011
 

Dellisanti, C.D., Raspanti, J.M., Grant, V.A., Hanson, S.M., Ghosh, B., KLUG, C.S., Czajkowski, C. (2011) Site-directed spin labeling electron paramagnetic resonance spectroscopy reveals proton-induced movement of loop 9 in GLIC

► 53rd Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 2011
 

Konovalov, A., Raguz, M., Krpan, I., Subczynski, W.K. Physical Properties of Membrane Models of the Eye-Lens Fiber-Cell Plasma membrane for Animals of Different Life Spans: Saturation-Recovery EPR Spin-Labeling Studies.

R. R. Mett, J. W. Sidabras, and J. S. Hyde. Hyperbolic Cosine Waveguide Tapers and Oversize Rectangular Waveguide for Reduced Broadband Insertion Loss in W-band EPR Spectroscopy. (PPTX)

R. R. Mett, J. R. Anderson, J. W. Sidabras, T. S. Thelaner, and J. S. Hyde. Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band: Practical Considerations. (PPTX)

J. W. Sidabras, R. R. Mett, J. R. Anderson, and J. S. Hyde, “Resonator Advances for W-band Pulse Spectroscopy,”

J. W. Sidabras, R. R. Mett, S. K. Varanasi, H. M. Swartz, and J. S. Hyde, “Microwave Surface Resonator Array for In Vivo Electron Paramagnetic Resonance Spectroscopy.”

► Neuroscience 2011, Washington, DC | November 12-16, 2011
 

Dellisanti, C.D., Raspanti, J.M., Grant, V.A., Hanson, S.M., Ghosh, B., KLUG, C.S., Czajkowski, C. (2011) Site-directed spin labeling electron paramagnetic resonance spectroscopy reveals proton-induced movement of loop 9 in GLIC.

 2010

Talks
 

► APS March Meeting, Portland, OR | March 15-19, 2010
 

L. Mainali, I. D. Sahu, K. A. Earle, “EPR Data Analysis on Copper Complexes using Bayesian Inference, Nonlinear-Least Squares and Statistical Geometry

Sahu, L. Mainali, K. A. Earle, “EPR Spectral Analysis of Vanadyl Model Compounds via Bayesian Inference, Nonlinear-Least Squares and Statistical Geometry

► Marquette University College of Engineering | April 9, 2010
 

R. R. Mett. Exponential Waveguide Taper and Oversize Waveguide for Reduced Losses and Improved Signal-to-Noise Ratio in EPR Spectroscopy. (PPTX)

► Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico | May 2-6, 2010
 

Antholine, W. Multifrequency EPR of Cupric Sites: Four Coordinate, Three Coordinate, and Free Radical Like Sites. (PDF)

Bennett, B. Rapid-Freeze-Quench EPR: What, Why, and How. (PDF)

Feix, JB. Structure and Conformational Dynamics of the Pseudomonas aeruginosa Toxin , ExoU. (PDF)

Hyde, JS. Experiments at Low Microwave Frequencies. (PDF)

Joseph, J. Lopez M, Chen G, Prah D, Ramadoss J, Zielonka J, Kalyanaraman B. Mitochondria-Targeted Nitroxides: Synthesis and Biological Applications. (PDF)

Sidabras, J., Mett RR, Hyde JS. Rapid Development of EPR Resonators using Finite-element Modeling. (PDF)

Subczynski, WK. Ragua M, Mainali L. Physical Properties of Lipid Bilayers using Spin Label EPR. (PDF)

Vasquez-Vivar, J. Whitsett J, Yu L, Ji X, Tan S. Deficient Tetrahydrobiopterin Explains the Double-Hit of Fetal Brain Damage in Hypoxia-Ischemia: A Role for NOS Uncoupling. (PDF)

Zielonka, J. Detection of Peroxynitrite by Boronate-based Probes: EPR, Fluorescence and HPLC Studies. (PDF)

► VIII International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 5, 2010
 

Candice S, Klug, Effects of dysfunctional mutations on the function of the bacterial ABC transporter MsbA. Distance Measurements.

James S. Hyde. Pure Absorption Rapid Scan (PARS) EPR Spectroscopy.

Witold K. Subczynski. Spin-label oximetry at X-band, Q-band, and W-band.


Posters
 

► Annual Meeting of the Biophysical Society, San Francisco, CA | February 20-24, 2010
 

Gadicherla AK, Antholine WE, Camara AKS, Heisner JS, Aldakkak M, Stowe DF. Free radical generation and electron flux in mitochondrial Fe-S centers during cardiac injury: Changes with mitochondrial protective drug ranolazine.

Kaminski, H. and Feix, J.B. Biophysical Studies of Cecropin-Mellitin Antimicrobial Peptides with Improved Selectivity.

Westfahl, K.M., Merten, J.A., Klug, C.S. Characterization of the L511P and D512G mutations in the MsbA lipid flippase.

► Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico | May 2-6, 2010
 

Kittell AW, Sidabras JW, Hyde JS. Measuring Dipolar Distances Using Low Frequency (1.8 GHz) Continuous Wave EPR. (PDF)

Westfahl KM, Merten J, Klug CS. Characterization of the H537A Mutation in the ABC Transporter MSBA. (PDF)

Zielonda J, Sikora A, Lopez M, Joseph J, Kalyanaraman B. Detection of Free-Radical Intermediates and Products derived from the Reaction Between Boronates and Peroxynitrite-EPR Spin-Trapping and HPLC Studies.

► 52nd Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 2010
 

Mainali, L., Raguz, M., and Subczynski, W. K. New approaches in discrimination and characterization of membrane domains using EPR spin labelling methods.

Mainali, L., Raguz, M., and Subczynski, W. K. Membrane domains in sphingomyelin /cholesterol membranes: Their structure and properties using EPR spin labelling methods.

R. R. Mett, J. R. Anderson, J. W. Sidabras, and J. S. Hyde. Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band. (PPTX)

J. W. Sidabras, R. R. Mett, and J. S. Hyde, “Advances in Loop-Gap Resonator Technology for X-band Aqueous Samples.

► Annual P-41 Directors Meeting Bethesda, MD | 2010
 

Hyde JS, Kittell AW, Camenisch TG. Nitroxide line-shape analysis with X-band pure absorption rapid scan (PARS) electron paramagnetic resonance (EPR).

► MCW Research Poster Day, Milwaukee, WI | September 15, 2010
 

Zhuo, Y., Zhan, Z., Gurevich, V.V., Klug, C.S. (2010) Identification of interaction sites in arrestin3 upon binding to ERK2 and JNK3.

Westfahl (Schultz), K.M., Merten, J.A., Klug, C.S. (2010) Characterization of Dysfunctional Mutations in the ABC Transporter MsbA

► VIII International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 2010
 

Widomska, J., Raguz, M., Subczynski, W. K. Oxygen transport in lipid bilayer membranes.

Raguz, M., Mainali, L., Widomska, J., Subczynski, W. K. Spin-label oximetry in discrimination and characterization of cholesterol crystalline domain.

Wisniewska, A., Widomska, J., Subczynski, W. K. The liquid-ordered-like phase in xanthophyll containing membranes as examined by pulse-EPR oxygen probing.

 2009

Talks

► Milwaukee School of Engineering | September 2, 2009

R. R. Mett, J. D. Gassert, and R. A. Strangeway, "EPR at MCW," faculty inservice presentation

► Acert Workshop Multi-frequency ESR/EPR, Cornell University, Ithaca, NY | November 15-17, 2009

Subczynski, W. K. Three-dimensional dynamic structure of lipid bilayer membranes saturated with cholesterol. Promising advances at Q- band and W-band


Posters

► Biophysical Society Annual Meeting

Impellitteri, N.A., Merten, J.A., Bretscher, L.E., Klug, C.S. Identification of functionally important sites within the cysteine-free inner membrane transferase protein ArnT.

Raguz, M., Widomska, J., Subczynski, W. K. Three-dimensional dynamic structure of phospholipid bilayer saturated with cholesterol.

► 51st Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 21, 2009

R. R. Mett, J. W. Sidabras, and J. S. Hyde, “X-Band 2-Loop-1-Gap LGR and Long-Slot Iris for Reduced Frequency Pulling”

R. R. Mett, J. W. Sidabras, and J. S. Hyde, “Use of Oversize Rectangular WR-28 Waveguide at W-band for Low-Loss and Increased Signal-to-Noise Ratio”

J. W. Sidabras, R. R. Mett, P. N. Lesniewski, H. M. Swartz, and J. S. Hyde, “Counter-Rotating Current Microwave Resonator for In Vivo EPR Spectroscopy”

► Annual P-41 Directors Meeting, Bethesda, MD

Bennett B, Kowalski JM, Antholine WE, Sidabras JW, Anderson J, Walter ED, Millhauser GL, Hyde JS. Development of EPR of Cu(II) at 1 - 3 GHz for the study of proteins involved in neurodegenerative diseases.

Hyde JS, Froncisz W, Camensich TG, Strangeway RA. EPR correlation spectroscopy using rapid radio frequency sweep at W-band.

Contact Us

National Biomedical EPR Center
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Medical College of Wisconsin
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Director and Professor of Biophysics
jshyde@mcw.edu

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Administrative Assistant
smorton@mcw.edu

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